جۆری توێژینه‌وه‌: Original Article

نوسه‌ران

1 Department of Medical Laboratory Techniques, AL-Maaref University College, Ramadi, Al- Anbar, Iraq

2 Department of Chemistry, College of Science, University of Anbar

3 Biotechnology Research Center, University of AL-Nahrain

پوخته‌

Caraway (Carum carvi L.) is an important medicinal plant that contains a range of bioactive compounds. The extraction process was carried out using four separate solvents: 70% methanol, 70% ethanol, 70% hexane, and water by continuous extraction using the Soxhlet device, has been chemical detection of effective ingredients in crude extracts. The volatile oils were also extracted by the aqueous distillation method using the Clevenger device and the chemical compounds found there were detected using GC-Mass technique. The study has been effect of active Components for the crude extracts and oil extracted for the seeds of the Caraway plant on the growth of the L20B,SR cancerous cellular lines out of the living body (in vitro) using two concentrations (12.5, 25)µg/ml within a 72-hour exposure period. The results showed a incorporeally difference and a probability level of P < 0.05 for some crud extracts. Where the highest rate of inhibition on the cancerous cell line L20B at the concentration of 12.5 μg/ml for ethanol extract of 29.7%, as for the SR cancerous cell line, it was the highest inhibition for extract Hot water at low concentration of 73.28%. The extracted oil also had an effect on the growth of the cancer cell lines under study. The objective of this study is to test the toxicity of crud extracts and oil to the seeds of the Caraway plant on the L20B and SR, and to know the amount of oil in the seeds of the Caraway plant

وشه‌ بنچینه‌ییه‌كان

1- Meena, A. K., Singh, B., Yadav, A. K., Singh, U., Kaur, R., Sachan, A., Gautam, V. & Pal, B.  (2010). Review on medicinal properties and bioactive constituents of herbal spices commonly used in India. Journal Pharm Res., 3(4), 866-868.

2. Keshavarz, A., Minaiyan1, M., Ghannadi, A. & Mahzouni, P. (2013). Effects of Carum carvi L. (Caraway) extract and essential oil on TNBS-induced colitis in rats.  Journal List Res Pharm Sciv, 8(1), 1-8.                                                                                                       

3- Begum, J., Bhuiyan, M. N., Chowdhury,  J. U.,  Nuzmul-Hoque, M., & Anwar, M.  N.(2008).   Antimicrobial activity of essential oil from of Carum carvi and its composition   Bangladesh. Journal Microbiol, 25(2), 85-89.                                                                             4- Nicola S. Iacobellis, Pietro L., C., Francesco C. & Felice S. (2005) . Antibacterial Activity    of Cuminum cyminum L. and Carum carvi L. Essential Oils.  Journal, Agric. Food Chem., 53, 57-61.                                                                                        

5- Johri, R. K . (2011) . Cuminum cyminum and Carum carvi. Jan-Jun, 5(9), 63–72 .

6-   Al-Janabi,  M. (2018) . Antibacterial  Activity Of Caraway seeds and Indian costus roots essential oils.  G.J.B.B., 7 (1) , 44 - 48.

7- Katarzyna S., Ł., Bogdan K., Elżbieta K. & Jan B.(2013).  Microbiological activity of caraway (Carum carvi L.) essential oil obtained from different origin. Journal, Acta Scientiarum, 35, 4, 495-500.

8- Sedlakova, J., Kocourkova, B., Lojkova, L. &  Kuban, V. ( 2003) .  The essential oil content in caraway species (Carum carvi L). Hort . SCI. (PRAGUE), 30(2), 73–79.

9- Freshney, J. M. (2001). Application of cell culture to toxicology. Cell Biology and Toxicology, 17, 213-230.

10 - Lopoz –Lazaro, M., Palma, D. I. , Pera, N., Pastor, N., Marlin-Cordero, C. , Navarro, E. , Corler, F., Ayso, M. J. & Toro, M.V.(2003). Antitumor activity of digitalis purpurea L. subsp. Heywoodii .Plant Med.,69,701-704.

11- Petraitytė, N., Dastikaitė, A. , (2007) . Agrobiological  assessment of wild Carum carvi Lcenopopulation biodiversity ex situ . Biologija, 53, 4 , 74–79 .                                                 12-  Kato , M. , Mizuno , K. , Fujimura , T. , Iwama , M. , Irie , M. , Crozier , A. & Ashihara , H. ( 1999). Purification and characterization  of caffeine synthase from tea leaves . Plant physiology, 12, 579 – 586.                                                                                                          

13- Kelmanson, J. E., Jager, A. K. & Staden, T. V. (2000) . Zulu medicinal plant with antibacterial activity. Journal Ethno pharmcal, 69, 241 – 246.

14- Eslah S. R., Khlood W. A. &  Zaynab Y. M. H. (2016 ) .Purification and Identification of Flavonoids Extracted from Loranthus Eurpaeus Fruits. Journal of Biotechnology Researches Center, , 10 (1), ,1815-1140.  

15- Bouaklin,  A., Lacroix, C. , Roux, N., Gangheux, J. P. & Deronin, F. (2000). Fungal contamination of food in Dermatology units. Journal, Clin . Microbilogy , 38 , 4272- 4273.

16- Melvinjoe, M. , Jayachiyral, J. &  viyayaprixa, M. (2000). Candida and Candisis. 3rded. Journal of medicinal and plant Research, , 3(11) ,1134- 1136.

     17-Mark, A. W., Richard, C. G., J. R. &  Martin, I. R. (1995). Prdispasing factors of systemic fungal in fections of genitourinary tract. Journal of Uholwy, 154, 160-163.                                                    

18- Roberts, M. & Wink, M. (1998). Alkaloids Biochemistry Ecology and Medicinal application. plenum press NewYork- London , 435-459.

19- Freshney, R.I., (2012). Culture of animal cell. 6nd ed., Wily- Liss, NewYork .

20- Allen, J., W., Schuler, C., F., Mends, R., W. &  Latt, S., A., (2005). Asimplified technique for in vivo analysis of sister chromatid exchange using 5-bromodeoxy uridine tablest, cytogentics. ,18, 231-237.

21- Johri, R., K.  (2011). Cuminum Cyminum and Carum carvi:Anupdate. Pharmacogen Rev. , 5(9) , 63-72.

22- Johri G., K., Abdullah, S., S., Kumari,  P., S.  (2014). Antidiabetic Effect of Costus speciosus Rhizome Extract in Alloxan Induced Albino Rats.  Journal of chemistry and biochemistry , 2(1) ,13-22 .

23- Choudhury, N., Kalita, J., C. & Haque, A., (2012). " Effect Of Costus Speciosus Koen on Reproductive organs of  Female Albino Mice . Internationl Reseach journal of pharmacy , 3(4), 200 - 202.                                                                    

24- Chibueze, F., U., Kingley, N., A., Okorie, K., N., Agwu, A. &  Success, N., N. (2012). Phytochemical Composition Of Costus Afer Extract and its Alleviation of Carbon Tetrachloride-induced Hepatic oxidative Stress and Toxicity. International Journal of Modern Botany , 2(5) ,120-126 .

25- Nakayama, T., Hor, K., T. & Kawakish. S., (2009). Comparision of the Cytotoxicity Of different hydroperoxides to v79cell. Free Radical Res. Communications , 14(3) , 173-178.

26- Abdel-Massih, R.,  M.,  Fares, R., Bazz, S., EL-Chami, N. & Baydoun, E.(2009). The apoptotic and anti-proliferative activity of origanum majorana extracts on human Leukemic cell line. Leuk Research, 34(8), 41-48.

 27- Moon, Y., J., Wange, X. & Morris, M., E. (2006). Dietary flavonoids: effects on xenobiotic and carcinogen metabolism. Toixcal in vitro, 20(2) , 187-210.

 28- Omenu, G. S.(1995).What accounts for the association of vegetables and fruits with lower incidence of cancers and coronary heart diseases . Ann Epideimal., 5(4), 333-5.

29- Sadeghi, H. & Yazdan, P., R. (2003). Anti-tumor activity and cell cycle arrest of a new diterpene ester from Dophne mucronala using K562 cell. Biomed Journal, 7(3),127-131.

  30- Salimon K.M, & Badeaa, R.I., (2002). Effect of oil Extracted from some medical plants on differe mycotoxigenic fungi. Journal Food chem. Toxicol, 40(11), 1669 – 1675.                                              31- Locobe LLi , N., Contro, S., Capposso, P. & Sehator, F. (2005). Anti bacterial activity of  Cuminum Cyminum L, and  corum Carvia L., essential oils . Journal Agric food  chem. , 53(1), 57 – 61. 

32- Fabio,  A., Corona,  A, Forte, E. & Quaglio, P.  (2003). Inhibitory Activity of spices and essential oils of psychrophilic Bacterial . Journal ,The new Microbiologica, 26(1), 115 – 120 .

33- Wienck, A., Boeck, I.,  Eisold,  S.,  Klar , E. & Linnebacher, M. (2009). Identifizierung eines immunogenen T-Zell-Epitopes des MSI-Zielantigens U79260(FTO) . Journal Biomed  Biotechnd , 38, 29 – 30.

  34- Rimutė, M. (2012). Potenial pathogens of common caraway (Carum carvi L.) seeds and search for measures suppressing their spread. Journal Agriculture., 99, 2 , 179‒188.                35- Olatunde, A. & Dikwa, M. A. (2014). Qualitative and Quantitive Anylysis of Phytochemicals of Loranthus bengwensis Leaf. IntRJPharmSci, 5(1),10-12.                            36-  Thatte, U.,  Bagadey, S. & Dahanakar, S.  ( 2000). Modulation of programmed cell death by medicinal plants. Send to Cell Mol Biol (Noisy-le-grand)., 46(1) ,199-214.