جۆری توێژینه‌وه‌ : Original Article

نوسه‌ران

• Asaad M Mahmood1 ¹
• Jim M Dunwell ²

¹ 1Department of Biology, College of Education, University of Garmian, Kalar, KRG/Iraq

² School of Biological Sciences, University of Reading, Reading, Berkshire, UK

پوخته‌

2-oxoglutarate-dependent (2-OG) dioxygenases [2(OG)-dioxygenases] are distributed in a wide
range of prokaryotes and eukaryotes, and are involved in many different important biological
activities, for example biosynthesis of plant products including plant hormones and antioxidants,
posttranslational modification, DNA/RNA damage repair and in certain organisms modulations
of epigenetic modifications. These 2(OG) dioxygenase enzymes contain a characteristic iron (Fe)-
binding active site. To understand their role in modulating mechanisms against unfavourable
conditions, the present study examined nuclear localization signal (NLS), genetic status,
phenotypic patterns of 14 T-DNA mutants of Arabidopsis containing an insert in their (2-OG)
dioxygenase genes. Results showed that predicted localizations of proteins encoded by
respective genes varied according to four different methods used in this analysis; seven, three,
one and one of them were predicted to be located in the nucleus according to the four methods,
respectively. Moreover, genotypic analysis confirmed the homozygosity of plant(s) of nine of
these mutant lines [N671573 (insert in At1g20270), N668172 (At1g68080), N652869
(At2g17720), N679576 (At3g06290), N678627 (At3g28490), N338446 (At4g35810), N683883
(At4g35820), N666896 (At5g18900), N598611 (At5g66060)] and phenotypic analysis including
vegetative, siliques and seed characteristics of those plants showed different characteristics
either within each mutant (homozygous and heterozygous), or between mutants and the WT.