Journal of Garmian University

Molecular characterization of autochthonous grapevine (Vitis vinifera L.) cultivars in Kurdistan using simple sequence repeats (SSRs)

جۆری توێژینه‌وه‌ : Original Article

نوسه‌ران

Asaad Mahmood 1
Shaimaa H. Ali 2
Shabaq M. Hawezy 3

1 Department of biology, College of Education , University of Garmian

2 Scientific Research Center, College of Science, University of Duhok

3 Department of Horticulture, College of Agriculture, University of Sallahaddin

https://doi.org/10.24271/ garmian.196358
پوخته‌
Grapevine (Vitis vinifera L.) is one of the oldest and the most extensively grown fruit plants worldwide with regards to its great economic significance throughout the world. Northern part of Iraq (Kurdistan) is regarded as one of the regions in Iraq where grapevine plants have been taken into cultivation. Despite the economic importance of grapevine cultivars in this region, they have not been precisely classified and their genetic data is also limited. In the present study 12 simple sequence repeat (SSR) markers were used to evaluate the genetic diversity among 13 local grape cultivars, also determining their genetic relationships with four Eurpean ones (17 cultivars studied). Results showed that the observed number of alleles (N) per locus varied from 3 to 9 with an average of 5.25 and the values of polymorphism information content (PIC) ranged from 0.28 to 0.776. Despite the average value (0.1716) of observed heterozygosity (Ho) was less than the average level (0.6427) of expected heterozygosity (He), results were relatively demonstrated a random union of gametes in the population. In addition, genetic similarity among respective cultivars ranged from 0.17 to 0.1, indicating valuable genetic variation among studied cultivars. The analysis of UPGMA clustering demonstrated high genetic variation among those cultivars and grouped them into three main clusters. Findings from the present study might render striking information in determining local cultivars and in breeding management strategies that are of importance for genetic conservation and cultivar improvement.

وشه‌ بنچینه‌ییه‌كان

Vitis vinifera
Germplasm characterization
microsatellite
SSRs
Genetic diversity
Crossmark
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Journal of Garmian University
خول 6، ژماره‌ 3
هاوین 2019
لاپه‌ڕه‌ 328-335