جۆری توێژینه‌وه‌ : Original Article


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Transposable elements and other sequences of repetitive DNA including microsatellite are usually subject to both DNA methylation and transcriptional silencing. However, anti-silencing mechanisms which lead to promote transcription in such regions are not well investigated. A recent genetic screening in Arabidopsis thaliana identified an anti-silencing factor, named Bromodomain and ATPase domain-containing protein 1 (BRAT1). This protein is involved in DNA demethylation through a valuable association between histone acetylation and transcriptional anti-silencing at methylated genomic loci. This involvement can be conserved in eukaryotes. Although protein acts as an anti-silencing factor, there is no previous study identifies its contribution in gene regulation under unfavorable conditions. This study was analyzed several molecular patterns of the respective gene including protein-protein interactions, Nuclear Localization Signals (NLS), Cis regulatory elements (CREs) and intron-mediated enhancement (IMEter) using recent bioinformatic data bases. Results showed protein-protein interactions between the respective gene product and other proteins are involved against abiotic stresses, the protein of this gene is localized in nucleus. Results were also observed several CREs of non-coding regions representing their roles as stresses-responsive factors, according to IMEter analysis, this response is expected to valuably present in Intron 1, suggesting experimental studies on mutant lines that contain insertions in their non-coding regions specifically intron 1of the underlying gene.

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