Iraq one of the most Middle East countries confronts to climate change, where it faced many environmental challenges the most important of high temperatures above the rate of the natural. The Nitrous oxide(N2O) gas work on the absorption of part of the heat inside the atmosphere and prevent the leakage into space and this leads to increase temperature of the air and knows this phenomenon of global warming, N2O has another effect on the atmosphere as it works to drain the ozone layer that protects the atmosphere from harmful ultraviolet rays. The aim of this research is to study the effect of N2O to increase their concentration in the atmosphere of temperature variation. Where the data was taken from satellites recorded by the European Centre for medium range weather forecasts (ECMWF) . This study choice Iraq and neighbor regions from latitude 27 to 37 ºN and longitude from 39 to 50 ºE for years (2003-2016), where we studied the behavior of N2O gas concentration and its relationship with air temperature variation. The result by using correlation coefficient between N2O and temperature it found that the relationship was high positive in the all stations of study, where in Sulamaniyah (Iraq) +0.8 , and also be a strong positive relationship in southern stations, as for neighboring regions also be high positive in Batman (Turkey) +0.7, and Amman (Jordan) +0.8 also in Aleppo(Syria) +0.8 ,and in Jubail (K.S.A) correlation coefficient +0.8, and in Ahwaz (Iran) be +0.8 .