جۆری توێژینه‌وه‌: Original Article

نوسه‌ر

Veterinary Directorate of Garmian, Kalar, Kurdistan Region, Iraq

پوخته‌

This study was conducted to explore the role of pumpkin seed oil (PSO) ofcucurbitapepo ,as alternative of traditional medicinal treatment for manage wound healing in mice .Firstly, the oil of cucurbitapepo (pumpkin seed oil) obtained by hot extraction method with n-hexane solvent .Forty male Balb- c mice were coetaneous induced surgical wound on the back region bilaterally .Theywere divided into four equal groups and treated topically as following . Group one (G1) treated with pumpkin seed oil, group tow (G2) treated with MEBO, group three (G3) treated with combination of pumpkin seed oil plus MEBO. While group four (G4) didn’t received any treatment and considered as control. Clotting time, platelet count, blood glucose, insulin activity, white blood cells count, red blood cells count, differential white blood cells count, pro thrombin time, thrombin time and fibrinogen level, were assessed after one day of induced wound and after complete wound healing in all experimental animals. The wounds were followed up through measuring their diameters, photographically and histological (through obtaining skin biopsy after 4, 6,8,10daysand so on of incision tillhealing. There were no statisticaldifferences at p<0.05 in clotting time, platelet count, red blood cells count and pro thrombin time between the values measured after one day of incision when compared to the values measured after healing in all experimental groups. The diameter of wounds decreasesi.e. wound healed proportionally with the time of treatment. Histological appearance of the wound of experimentalgroupsexhibited progression in wound healing which was taking4-10 days after treatment. All healed wounds of various groupsshamed decrease in inflammatory cell with collagen infiltration in the dermis and complete regeneration of the epithelial cells in the epidermis.

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