جۆری توێژینه‌وه‌: Original Article

نوسه‌ران

پوخته‌

Five clinical isolates of Trichophyton rubrum isolated from patients with
dermatophytosis in the Dermatology unit of the General Hospital in Kalar distric/Sulaimani
province/North region of Iraq. The isolates were identified according to the conventional
laboratory methods and molecular methods, one of these strains was isolated from the groin
of an infant with one year old and identifies as Trichophyton rubrum var. raubitschekii. This
variety characterized by the production of cottony to a velvety colony. The front of the colony
appeared brown color with brownish pigmentation at the reverse. Slide examination revealed
numerous cylindrical shaped microconidia and few longer macroconidia. Molecular
identification was conducted according to the conventional PCR by using set primers ITS1
and ITS4 and resulted in PCR product about 690bp. PCR-RFLP by using BstN1 digestion
enzyme revealed three pattern bands 380,180,100bp. While sequencing of the ITS region
revealed high similarity with that of a number of uncultured endocytic fungus clone.

وشه‌ بنچینه‌ییه‌كان

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