جۆری توێژینه‌وه‌: Original Article

نوسه‌ران

1 Department of Horticulture and Landscape Design, College of Agriculture University of Basra, Basra, Iraq

2 Department of Horticulture and Landscape Design, College of Agriculture University of Basra, Basra, Iraq

پوخته‌

Results showed that fresh and dry weight of primary and embryogenic callus
increased significant with increasing of casein or paclobutrazol after 16 weeks from
culture. The treatments of 1000 mg.L-1
casein hydrolysate and 0.5 mg.L-1
paclobutrazol showed fewer periods to embryo formation reached 21.00 and 17.00
days, respectively. However, the control observed the longest period to somatic
embryo formation which reached 61.00 days. The treatments of 1000 mg.L-1
casein
hydrolysate and 1.0 mg.L-1
paclobutrazol showed the highest values 6.50 and 1.60
cm length, 0.87 and 0.80 cm diameter, 0.336 and 0.361 gm fresh weight and, 0.022
and 0.033 gm dry weight of embryo’s characteristics, respectively. In addition, two
treatments of paclobutrazol (0.5 and 0.7 mg.L-1
) showed high average number of
embryos (16.33 and 16.67 embryos, respectively). In contrast, the recorded data
from the control treatment gave demonstrated fewer values (4.33 embryos, 0.80 cm
length, 0.13 cm diameter, 0.035 gm fresh weight and 0.005 gm dry weight) of
characteristics of embryos. When the culture medium contained casein hydrolysate
at 1000 mg.L-1
observed the highest percentage of germination (76%) and less
period of embryo germination (15.30) days in comparison with other treatments.
Moreover, the culture medium with low concentration of paclobutrazol (0.3 mg.L-
1
), showed the highest percentage of germination (75.67%) in comparison with
other treatments of paclobutrazol. However, the same concentration of
paclobutrazol obtained the lowest period of embryo germination (14.0 days) in
comparison with the other treatments. The results also showed that the callus when
cultured on MS medium supplemented with 800 or 1000 mg.L-1
casein hydrolysate
demonstrated indirect adventitious shoots.

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